New laboratory criterion for the differential diagnosis of sarcoidosis and pulmonary tuberculosis

New laboratory criterion for the differential diagnosis of sarcoidosis and pulmonary tuberculosis
Автор:  Starshinova A., Malkova A., Zinchenko U., Basantsova N.,Kudryavtsev I., Lapin S., Mazing A., Surkova E., Pavlova M. Belyaeva E., Stepanenko T., Yablonskiy P.
Год:  2020
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Ключевые слова: tuberculosis, sarcoidosis, differential diagnosis, diagnostic criteria


Take-Home Message. This study provides a new diagnostic index based on the antibodies to mutated citrullinated vimentin concentration and the level of different B subsets. Determination of index over 5 Units proved to be characteristic of sarcoidosis, what might help to differentiate SD from TB.

Abstract. In some cases there is a problem of differential diagnosis of sarcoidosis(SD) and tuberculosis(TB)because of the similarities in clinical, X-ray and laboratory features. The aim of this study was to search for new differential diagnostic criteria for sarcoidosis and tuberculosis by calculating the index, based on the level of autoantibodies to modified citrullinated vimentin (anti-MCV) and the level of B-cell subpopulations. These parameters were measured in patients with sarcoidosis (n = 93), tuberculosis (n = 40) and healthy donors (n = 40) using the ELISA and cytometry. The absence of a statistically significant difference when comparing the level of anti-MCV, the number of B-cells in SD and TB suggests that these changes may be characteristic of granulomatous diseases. The use of the formula Ds=([B-naïve%]\[B-memory%])*([B-CD38%]+[B-CD5%])/[anti-MCV] might allow to differentiate SD with an increase in the calculated index of more than 5 units with a sensitivity of 80.00% and specificity of 93.10% (AUC = 0.926).

Starshinova, Anna & Malkova, Anna & Zinchenko, Ulia & Basantsova, Natalia & Kudryavtsev, Igor & Lapin, Sergey & Mazing, Alexandra & Surkova, Elena & Pavlova, Maria & Belyaeva, Ekaterina & Stepanenko, Tatiana & Yablonskiy, Piotr. (2020). New Laboratory Criterion for the Differential Diagnosis of Sarcoidosis and Pulmonary Tuberculosis. DOI:10.21203/